Figure 2. Immunoblot analysis of LepRb and SOCS3 and Jak2/Stat3 signaling in hypothalamus from naïve young (NY), SCI young (SCIY), naïve aged (NO) and SCI aged (SCIO) mice.

LepRb expression is significantly decreased in SCIY, NO and SCIO animals when compared to the NY control. Similarly, Jak2 phosphorylation and Stat3 phosphorylation are both attenuated in SCIY, NO and SCIO when compared to the NY control. Conversely, the expression level of SOCS3 is significantly elevated in SCIY, NO and SCIO when compared to the NY control. Jak2^Total and Stat3^Total were used as internal standards. β-Actin was used as a protein loading control. Statistics are according to data analysis methods described. p≤0.05. n = 8 for each group.