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. 2012 Jul 16;7(7):e39794. doi: 10.1371/journal.pone.0039794

Figure 7. Eae18b regulates intrathecal production of CCL11 and influences the BBB integrity.

Figure 7

(A) During the whole disease course CCL11 protein levels were increased in the cerebrospinal fluid (CSF), in the congenics (Eae18b) compared to controls (DA). Experiments repeated twice, three to five animals per group analyzed. (B) mRNA levels of Ccl11 measured in the late disease time point (day 23 p.i.) showed upregulation in the congenic spinal cord. Error bars represent SEM, and (*) indicates p-value <0.05, (**) indicates p-value <0.01, (***) indicates p-value <0.001 calculated by Student’s t-test in (A) and by Mann-Whitney non-parametrical test in (B). (C) Representative images of occludin, a marker for the BBB tight junction components; more abundant in the congenic spinal cord (cross sections of seven congenic and eight control animals analyzed on day 12 p.i.). (D) Representative image of CCR3, the main receptor for CCL11, expressed on ependymal cells of the spinal cord canalis centralis, including a detail in high magnification (160×). Five congenic and four control animals analyzed on day 15 p.i.