Figure 1. Multiple sequence alignment showing shared and group-specific motifs between the Agouti-like sequences.

We include sequences that are previously published or already known for AgRP and ASIP-like clusters. The alignment includes the newly identified Agouti-like sequences in arthropods and in fungi. The important C-x(6)-C and the C-x(5,6)-C motif regions are shown above the alignment. The sequences that have the C-x(6)-C and C-x(5)-C motifs are distinguished with red and black dotted rectangular boxes, respectively. The residues that are conserved between the novel sequences and the different groups are shown with black rectangular boxes. The sequences that belong to different lineages are indicated with the colored line segments at the left of the alignment.