Figure 5. Tumor-derived secretory antigen can efficiently induce the intrathymic negative selection.
(A) At 14 days after inoculation of parental Col26 and Col26-OVA, or at 22 days after inoculation of Col26-sOVA, each developmental stage of thymocytes was determined on DO11.10 or DO11.10/CCR2−/− mice. Expression of DO11.10 TCR on total thymocytes is shown. (B) The numbers of DO11.10medium immature thymocytes (left graph) and DO11.10high mature thymocytes (right graph) were determined on DO11.10 or DO11.10/CCR2−/− mice bearing each tumor. Data represent mean ± SD from three independent experiments. *, p<0.01. (C) Intrathymic Treg differentiation in the tumor-bearing mice. Percentage of DO11.10highFoxp3+ Tregs is shown in each panel. Representative results from three (A and C) independent experiments are shown here.