Skip to main content
. 2012 Jul 16;7(7):e40981. doi: 10.1371/journal.pone.0040981

Figure 2. Regulation with C-terminal DD.

Figure 2

(A) Maps of inducible expression vectors. (B) To measure expression in transiently transfected parasites ring stage parasites were transfected and the indicated Shld-1 concentrations were added the next day and maintained for 24 hours. Luciferase expression is represented relative to the positive control pEF-Luc kept without Shld-1. Results indicate the average of 4 experiments and error bars show the standard deviation. (C) Luciferase activity on stably transfected parasites. Ring stage parasites of stable transfected lines were incubated for one day with the indicated Shld-1 concentrations. The measured luciferase activity was normalized by the number of parasites and expressed as percentages to the control line 3D7 transfected with Luc-GFP kept without Shld-1. (D) GFP fluorescence of the parasites described in (C) was measured by flow cytometry and is expressed relative to the control line Luc-GFP kept without Shld-1. RLU – relative light units, FI – fold induction of reporter expression, MFI – mean fluorescence intensity.