Figure 4. Cytokine production by IRF7-deficient cDCs in vitro.

CD11c^hi cDCs were sorted from spleens of C57BL/6 and B6.Irf7 ^−/− mice and cultured for 24 hours in the presence of 10 µg/ml PAM₃CSK₄, 1 µg/ml LPS and 1000U/ml IFNα, or combinations thereof. Supernatants were assessed by ELISA for presence of IL-12p70 (A&C) or IL-10 (B&D). Data show mean concentration of cytokine from triplicate wells ± SEM and are representative of two experiments. ** = p<0.01 *** = p<0.001.