Figure 5. IL-10 dependent impairment in Th1 polarisation by IRF7-deficient cDCs.

Splenic CD11c^hi cDCs were purified from C57BL/6 and B6.Irf7 ^−/− mice and cultured for 3 hours in the presence/absence of 10 µg/ml PAM₃CSK₄. DCs were cultured at a 1∶2 ratio with CFSE-labeled CD4⁺ OTII.Rag2 ^−/− cells for 7 days. TCRβ⁺CD4⁺ T cells were assessed for production of IFNγ after in vitro restimulation from cultures containing unstimulated DCs (A&C) or DCs pre-activated for 3 hours with PAM₃CSK₄ (B&C). Dot plots in A and B are representative, C shows mean percentage of IFNγ⁺ OTII.Rag2^−/− T cells in indicated culture conditions ± SEM. Each condition contained 3 replicate cultures, using DCs purified and pooled from 8–9 individual mice of each genotype. ** = p<0.01 *** = p<0.001.