Figure 4. Effect of DRiP78 on Chemokine receptor plasma membrane localization.

a) Knockdown levels of DRiP78 expression with the DRiP78-targetting shRNA. 48 hours post-transfection with cDNAs encoding DRiP78 (WT or shRNA) and receptor constructs forming CCR5 homodimers or CXCR4-CCR5 heterodimers, cells were labeled with biotin and precipitated using streptavidin. An immunoblot against GFP was then performed to show receptor levels at plasma membrane. c, and d) Quantification of at least 3 experiments, as performed in b). e) Plasma membrane expression of CCR5 WT or F/A in presence of DRiP78 WT or shRNA. f) Fluorescence measurements of cell surface expression of HA-CCR5 WT and F/A using an ALEXA-488 coupled secondary antibody.