Figure 5. DRiP78 levels in cells affect function.

a) Increasing amounts of DRiP78 cDNA were transfected in HEK293 cells and expression levels of chemokine receptor dimers were measured. b) Cells expressing chemokine receptor dimers were transfected for 48 hours with various amounts of cDNA encoding DRiP78 and fluorescence levels were then measured. Fluorescence appears only when receptors dimerize therefore any variation in fluorescence level would be attributed to a change in the capacity of those receptors to associate. c) Quantification of Rluc levels in cells, when expressed along with constructs mentioned in b). Rluc levels do not change, so fluorescence levels are not attributed to changes in the cell capacity to produce the proteins.