Figure 6. DRiP78 regulates CCR5 interaction with G protein subunits during assembly.
a) HEK293 cells were co-transfected with cDNAs encoding DRiP78 WT, HA-CCR5 WT or F/A, and various G protein subunits (Gαi-Rluc, Gβ1-Rluc in presence of Gγ2. Cells were then lysed and immunoprecipitations using an antibody directed against HA were performed. An immunoblot was then performed using an antibody directed against Rluc to reveal the G protein subunits. b) Histogram representation of the results obtained by immunoblotting. c) and d) BRET experiment showing WT CCR5-GFP10 or CCR5 F/A-eGFP interaction with Rluc-tagged Gαi, Gαs and Gβ1 subunits. * = p<0.05; ** = p<0.01 compared with negative controls. Results are representative of 3 independent experiments.