Figure 1. Ufo1 forms a homodimer via its UIMs.

A. ^GSTFL-Ufo1, ^GSTUfo1-WD40 domain, ^GSTUfo1-UIMs or GST beads were incubated with yeast extract from cells expressing full-length pGAL-GFP-UFO1 or pGAL-GFP-UFO1Δuims. The bead fraction was analysed by Western blotting with anti-GFP and anti-GST antibodies. T is 10% of yeast extract with which the beads were incubated. *denotes contaminant band. B. Recombinant ^GSTUfo1-UIMs or control GST beads were incubated with yeast extract with ^GFPUfo1-UIMs and analysed as above. T is 10% of yeast extract as above. C. Recombinant ^GSTUfo1 WD40 domain protein or control GST on GSH beads were incubated with bacterial lysate from cells that expressed ^HISUfo1-WD40 and the bead fraction was analysed by Western blotting initially with anti-HIS and then with anti-GST antibodies. T is 10% of yeast extract as above. The brackets around ^HISUfo1-WD40 in the anti-GST Western blot indicate that these bands were observed after incubation with anti-HIS antibodies as shown in the upper part of the blot.