Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Jul 16;7(7):e39210. doi: 10.1371/journal.pone.0039210

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Voloshin et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

Bacterial lysates from cells that produced ^HISRpn1 or ^GSTUfo1 were mixed with yeast extracts with ^mycCdc53 with or without ^GFPHo and divided into equal aliquots. Each aliquot was immunoprecipitated with a different antibody: anti-myc, anti-GFP, anti-GST or anti-HIS, in the presence of Protein A beads. The bead fractions were analysed by Western blotting with each antibody. + Ho: Lanes 1–3: Lane 1. 10% of the total (T) extract from yeast that produced ^mycCdc53, ^GFPHo and endogenous Ddi1; Lane 2. Bacterial lysate with ^GSTUfo1; Lane 3. ^HISRpn1 bacterial lysate; Lane 4. anti-myc immunoprecipitation; Lane 5. anti-GFP immunoprecipitation; Lane 6. anti-GST immunoprecipitation; Lane 7. anti-HIS immunoprecipitation. – Ho: Lane 8. 10% of the yeast extract with ^mycCdc53 and endogenous Ddi1 and bacterial lysate with ^GSTUfo1, Lane 9. 10% of bacterial lysate with ^HISRpn1. Lane 10. anti-myc immunoprecipitation; Lane 11. anti-GST immunoprecipitation; Lane 12. anti-HIS immunoprecipitation;The IP lanes are headed by the antibodies used for immunoprecipitation of each protein.