Figure 7.

The effect of R5P treatment on the action of Tkt RNAi. (A) Schematic diagram of the experimental strategy. After microinjection of Tkt dsRNA, the oocytes were cultured in M16 medium supplemented with 3-Isobutyl-1-methylxanthine for 8 hours for complete knock down of Tkt followed by a 16 hours culture period in M16 medium containing R5P. (B) Maturation rate in the presence and absence of R5P (^ap<0.05 vs. Tkt RNAi). The experiments were repeated at least three times (C) RT-PCR analysis showing changes in the expression of enzymes involved in the pentose phosphate pathway by R5P treatment. R5P, ribose-5-phosphate; Tkt, transketolase; Rbks, ribokinase; Prps1, phosphoribosyl pyrophosphate synthetase 1; RT-PCR, reverse transcriptase-polymerase chain reaction; GV, germinal vesicle; MI, metaphase I; MII, metaphase II.