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. 2012 Jun 30;39(2):87–93. doi: 10.5653/cerm.2012.39.2.87

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Copyright © 2012. The Korean Society for Reproductive Medicine

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Reverse transcription-polymerase chain reaction (RT-PCR) analysis of the selected genes after microarray analysis of the Lin28 knocked down mouse embryonic stem cells (mESCs). The expression of target genes selected by statistical method and filtering procedure after microarray analysis was examined by RT-PCR using specific primers with cDNA from control and Lin28 knocked down mESCs. Microphotographs representative of the results of (A) up-regulated and (B) down-regulated genes after RT-PCR are shown. Gapdh was used as a loading control. siCon, control siRNA transfected; siLin28, Lin28 siRNA transfected.