Fig. 2.

Immunofluorescence of synaptophysin in the hippocampal region of mouse brain in each developmental stage of embryonic (A), neonatal (B), and adult (C). (D) Magnified picture of (A). Note synaptophysin immunoreactivities connecting pyramidal cell layer (Py) and lacunosum molecular layer (LMol) through radiatum layer (Rad) (arrow). (E) Differential synaptophysin immunoreactivites between molecular layer (Mol) and oriens layers (Or) of hippocampal region in each developmental stages. ^*P<0.05. n=6-10. Nuclei are counterstained with DAPI. Scale bars=100 µm.