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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Virus Res. 2012 May 10;167(2):179–187. doi: 10.1016/j.virusres.2012.04.013

Fig.1.

Fig.1

Comparison of infection between WNV H8912 and WNV NY99 in mouse macrophage cells. Mouse macrophage cells were infected with both WNV strains at an MOI of 1. A, Q-PCR was used to measure WNV infection in cell lysates. B-C, Supernatant was collected to measure viral titer (B) and plaque size (C) by plaque assay. D, Immunofluorescence photomicrographs of macrophage cells at day 4 after infection. Cells were stained with antibody to WNV antigen (green signal). Hoechst dye was used as a nuclear counterstaining (blue signal), and merged images are shown. **P < 0.01 or *P < 0.05 for NY99 vs. H8912. §§ P < 0.01 or §P < 0.05 for WNV-infected vs. mock. P values were calculated with a non-paired Student’s t test.