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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Virus Res. 2012 May 10;167(2):179–187. doi: 10.1016/j.virusres.2012.04.013

Fig. 2.

Fig. 2

Comparison of infection between WNV H8912 and WNV NY99 in mouse kidney epithelial cells. Mouse kidney epithelial cells were infected with both WNV H8912 and its parent strain WNV NY99 at an MOI of 1. A, Q-PCR was used to measure WNV infection in cell lysates. B-C, Supernatant was collected to measure viral titer (B) and plaque size (C) by plaque assay. ND: not determined. D, Immunofluorescence photomicrographs of mouse kidney epithelial cells at day 4 post-infection. Infected cells and controls were stained with antibody to WNV antigen (green signal). Hoechst dye was used as a nuclear counterstaining (blue signal), and merged images are shown. **P < 0.01 or *P < 0.05 for WNV NY99 vs. WNV H8912. §§ P < 0.01 or §P < 0.05 for WNV-infected vs. mock. ## P < 0.01 compared to day 1 post-WNV H8912 infection. P values were calculated with a non-paired Student’s t test.