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. Author manuscript; available in PMC: 2013 Jul 16.
Published in final edited form as: Chem Res Toxicol. 2012 May 14;25(7):1402–1411. doi: 10.1021/tx200513t

Fig. 2. Effects of Nano-Co and Nano-TiO2 exposure on ROS generation (A) and 8-OHdG levels (b) in A549 cells and the effects of ROS scavengers or inhibitors on Nano-Co-induced ROS generation (C).

Fig. 2

A549 cells were pretreated with 5 µM H2-DCFDA for 2 h prior to exposure to Nano-TiO2 or Nano-Co for 12 h (A). A549 cells were treated with Nano-Co or Nano-TiO2 for 12h and 24h, respectively. 8-OHdG level was assayed by OxiSelect™ Oxidative DNA Damage ELISA Kit. (B). For antioxidant experiments, NAC or catalase was added 2 h prior to adding H2-DCFDA and Nano-Co (C). A549 cells without treatment were used as controls. Values are mean ± SE of six experiments. * Significant difference compared with the control, p < 0.05; # significant difference from the same dose of Nano-TiO2-treated group, p < 0.05; ** significant difference as compared with Nano-Co alone, p < 0.05.