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. Author manuscript; available in PMC: 2013 Jul 16.
Published in final edited form as: Chem Res Toxicol. 2012 May 14;25(7):1402–1411. doi: 10.1021/tx200513t

Fig. 3. Effects of Nano-Co or Nano-TiO2 exposure on DNA double-strand breaks (DSBs) in A549 cells (A) and the effects of ROS scavengers or inhibitors on Nano-Co-induced DSBs in A549 cells (B).

Fig. 3

A549 cells were treated with 0, 5 and 15 µg/ml of Nano-Co or Nano-TiO2 for 12h, and DSBs was determined by alkaline single-cell gel electrophoresis (Comet) assay and the tail moment was used as an index of damage (A). Values are mean ± SE of three independent experiments. Three slides per concentration and 100 comets per slide were analyzed in each experiment. For antioxidant experiments, catalase (2,000 U/ml) or NAC (10 mM) was added 2 h prior to adding 15 µg/ml Nano-Co for another 12 h and Comet assay was performed (B). * Significant difference as compared with the control, p < 0.05; # Significant difference as compared with the same dose of Nano-TiO2-treated group, p < 0.05.