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. Author manuscript; available in PMC: 2013 Jul 16.
Published in final edited form as: Chem Res Toxicol. 2012 May 14;25(7):1402–1411. doi: 10.1021/tx200513t

Fig. 7. Effects of the specific ATM inhibitor, KU55933, on Nano-Co-induced increased phosphorylation of p53 and ATM, and increased expression of Rad51 and p53.

Fig. 7

A549 cells were pre-treated with an ATM inhibitor, KU55933 (10 µM) for 1 h prior to treatment with Nano-Co (15 µg/ml) for 12 h. Cytosolic protein (for Rad51 and β-actin) or nuclear protein (for p-ATM, ATM, p-p53 and p53) was extracted for Western blot. Cells without any treatments were used as controls. A is the result of a single experiment. B is normalized band densitometry reading averaged from three independent experiments ± SE of Western blot results. β-actin or Coomassie blue stained gel was used to verify the equality of lane loading. * Significant difference as compared with the control, p < 0.05; # Significant difference as compared with the Nano-Co-treated group, p < 0.05.