Skip to main content
. Author manuscript; available in PMC: 2013 Jul 16.
Published in final edited form as: Chem Res Toxicol. 2012 May 14;25(7):1402–1411. doi: 10.1021/tx200513t

Fig. 8. Effects of ROS scavengers or inhibitors on Nano-Co-induced phosphorylation of ATM and p53, and increased expression of Rad51 and p53.

Fig. 8

A549 cells were pre-treated with Catalase (2,000 U/ml) or NAC (10 mM) for 2 h prior to exposure to 15 µg/ml of Nano-Co for another 12 h. Cells without treatment were used as controls. Cytosolic protein (for Rad51 and β-actin) or nuclear protein (for p-ATM, ATM, p-p53 and p53) was prepared for Western blot. Total ATM (for p-ATM), β-actin (for Rad51) or Coomassie blue stained gel (for p-p53 and p53) was used to verify the equality of lane loading. A is the result of a single experiment. B is normalized band densitometry reading averaged from three independent experiments ± SE of Western blot results. * Significant difference as compared with the control, p < 0.05; # Significant difference as compared with the Nano-Co-treated group, p < 0.05.