Table 3.
Primers used in this study
| Putative gene function | Forward primer | Reverse primer | Fragment length (bp) | GenBank Acc. |
|---|---|---|---|---|
| PR-1b family | 5' -TGCCCCTTCAGGTAAATCGT-3' | 5' -GCGGGTCGTAGTTGCAGATAA-3' | 125 | FY841927 |
| PR-2 family (Beta-1,3-glucanase) | 5' -CGACAACATTCGCCCCTTCT-3' | 5' -CTGCAGCGCGGTTTGAATAT-3' | 130 | FY843702 |
| PR-3 family (class I chitinase) | 5' -ACCTACAGCGCCTTCATTGC-3' | 5' -TGTGGTTTCATGCGACGTTT-3' | 120 | FY841849 |
| PR-3 family (class Iv chitinase) | 5' -CCATCGAAGCCCAGGTAATTT-3' | 5' -AGCCGGGAAGCAATATTATGGT-3' | 90 | FY843470 |
| PR-4 family | 5' -CCCCGTTACTGTCAATTGCAT-3' | 5' -AAAGCGTGACGGTGCGTATT-3' | 90 | FY841704 |
| PR-5 family (thaumatin-like) | 5' -GAACCAGTGCCCATACACAGTCT-3' | 5' -CCTGCGGCAACGTTAAAAGTC-3' | 96 | FY842709 |
| PR-6 family (proteinase inhibitor) | 5' -TGCTGGCGGCATCTATTTTA-3' | 5' -TAACACCTGCGCAAATGCA-3' | 90 | FY843534 |
| PR-9 family (peroxidase) | 5' -ACACCACCGTGCTGGACATT-3' | 5' -GTGCGGGAGTCGGTGTAGAG-3' | 118 | FY842918 |
| PR-10 family (ribonuclease-like) | 5' -TGTCTCAAGTGGAGGCAAGGA-3' | 5' -AAGCGACAATTTCAGGCAAAAC-3' | 90 | FY842956 |
| Antimicrobial peptide | 5' -GCGTTGCTCATACCCGTTTT-3' | 5' -GCAGCACTTAGCACTGGATGAA-3' | 90 | FY841562 |
| Cytochrome P450 | 5' -AACATGTCCTGCAGCACGAA-3' | 5' -GTGCACCGCAAGTAAACCAA-3' | 95 | FY841345 |
| Extensin | 5' -CGAATGTAATTCCGAAGTTGCA-3' | 5' -CCATCCCAAACCACCAGTCT-3' | 110 | FY844277 |
| Heat shock protein 70 | 5' -AACACCACCATTCCCACCAA-3' | 5' -CGAATTTGCCGAGCAGGTTA-3' | 130 | FY841300 |
| Hydroxyproline-rich glycoprotein precursor | 5' -GAGAAACTGGCACCGTCTTAGGA-3' | 5' -ACCTCCCCCTCCATCTCACA-3' | 140 | FY843962 |
| Metallothionein-like protein | 5' -TCAGGCTGCTGCGTTATTTG-3' | 5' -TGTCAGCGCAGTCACAATTTG-3' | 120 | FY842178 |
| xyloglucan endotransglycosylase | 5' -TCTGCGCCCCTACTTTTCC-3' | 5' -AGCTGGGCGATTGATCATGT-3' | 121 | FY842425 |
| Elongation factor-1 alpha | 5' -GGGAAGCCACCCAAAGTTTT-3' | 5' -TACATGGGAAGACGCCGAAT-3' | 160 | FY842441 |
The putative functional genes from (a) to (h) were clearly discernible ESTs in susceptible libraries. The putative functional genes from (i) to (p) were clearly discernible ESTs in resistant trees. Elongation factor 1-alpha (EF1a) was used as the reference gene, and the data were calibrated relative to the transcript levels in resistant trees prior to nematode infection (at 0 dpi). The data are presented as the mean ± S.D. of three replicates. Means designed by the same letter did not significantly differ at P < 0.05 according to Tukey's HSD test