Figure 3.

Generation of human serum anaphylatoxins induced by Micrurus spp venoms. Samples (50 μl) of normal human serum (NHS), as complement source, were incubated with 50 μl of Micrurus spp venoms (50 μg) or PBS for 30 min at 37°C. The generation of the anaphylatoxins (C3a, C4a and C5a) in the serum samples was measured by Cytometric Bead Array. Data are representative for three separate experiments and expressed as mean of duplicates +/-SD; results were expressed as concentration of each anaphylatoxin per mL of human serum.