Table 2.
Validation results
| Analyte | Measuring Range (ng/ml) MPA: μg/ml n = 3 |
LOQ, CV (%) Accuracy (%) n = 5 |
Sample Conc. (ng/ml) MPA: μg/ml |
Intra Assay Precision (%) n = 5 |
Inter Assay Precision (%) n = 20 |
Accuracy (%) Intra/Inter Assay |
|---|---|---|---|---|---|---|
| Cyclosporine A | 2 - 1250 | 3.7 | 2 | 7.4 | 7.1 | 125/113 |
| r2= 0.997 | 104 | 100 | 2.3 | 2.9 | 104/106 | |
| 500 | 0.9 | 2.5 | 106/105 | |||
| Tacrolimus | 0.5 - 42.2 | 7.5 | 0.5 | 12.3 | 9.1 | 138/100 |
| r2= 0.998 | 108 | 10 | 3.7 | 4.9 | 116/99 | |
| 20 | 3.8 | 4.5 | 111/98 | |||
| Sirolimus | 0.62 - 49.2 | 4.6 | 1 | 12.0 | 11.5 | 125/107 |
| r2= 0.999 | 106 | 10 | 5.1 | 5.0 | 100/102 | |
| 20 | 5.0 | 4.9 | 108/102 | |||
| Everolimus | 0.53 - 40.8 | 6.4 | 1 | 14.7 | 12.5 | 89/90 |
| r2= 0.999 | 118 | 10 | 2.9 | 6.9 | 97/96 | |
| 20 | 5.1 | 5.7 | 102/97 | |||
| MPA | 0.01 - 7.5 | 7.0 | 0.02 | 11.0 | 9.8 | 97/102 |
| r2= 0.999 | 105 | 0.2 | 1.3 | 3.2 | 97/97 | |
| 5 | 1.6 | 3.0 | 102/99 | |||
(LOQ: low limit of quantification, CV: variation coefficient)