Table 3.

Calibration results (Intra Assay Precision)

Analyte	Target Value (ng/ml) MPA (μg/ml)	Measured Value (ng/ml) MPA (μg/ml)	Accuracy (%)	Linear Regression (1/x weighting)
Cyclosporine A	0	< 0	N/A	y = 0.00419 × + 0.000559
	23.5	24.5	104	r = 0.9996
	127	121	94.9
	299	291	97.3
	484	506	105
	703	714	102
	896	877	97.8
Tacrolimus	0	< 0	N/A	y = 0.145 × + 0.00678
	2.1	2.45	117	r = 0.9994
	5.8	6.06	105
	12.1	12.3	102
	18.1	18.7	103
	24.4	24.2	99.3
	42.4	41.1	97.0
Sirolimus	0	< 0	N/A	y = 0.104 × + 0.0171
	2.6	2.25	86.7	r = 0.9996
	6.6	6.21	94.2
	12.8	13.0	102
	20.0	20.8	104
	29.0	28.9	99.5
	49.2	49.1	99.7
Everolimus	0	< 0	N/A	y = 0.112 × + 9.76e-0.09
	2.2	2.25	102	r = 0.9992
	5.9	5.66	95.8
	11.6	11.2	96.3
	17.0	18.0	106
	23.2	24.0	103
	40.8	39.6	97
MPA	4.27	1-point calibration linear through zero		y = 0.00494 x r = 1.0