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. 2012 Apr 17;4(1):163–167. doi: 10.3892/ol.2012.682

Figure 1.

Figure 1

BCL-2 gene knockdown by siRNA. (A) Quantitative real-time RT-PCR analysis of BCL-2 gene transcripts in SMMC-7721 cells. The experiment was performed 24 h following siBCL-2 (100 nmol/l) transfection with or without vitamin K2 (40 μM) infection. GAPDH was used as the internal control in the calculation. *P<0.05 as compared with the negative control. (B) Western blot analysis of BCL-2 protein in SMMC-7721 cells. The experiment was performed 48 h following siBCL-2 (100 nmol/l) transfection with or without VK2 (40 μM). Western bands were scanned and normalized over the internal control β-actin.