Figure 4.

Effects of glucose and serum withdrawal on CD133 expression. CD133^neg and CD133^hi cells were plated in 6-well plates with normal medium. After one day the medium was changed to DMEM plus 5% FBS (Control), DMEM with no serum (No serum) or glucose-free DMEM with 5% FBS (No glucose). The cells were harvested five days later and CD133 expression was monitored by FACS. Solid purple, CD133 reactivity of CD133^neg cells; green overlay, negative control; red overlay, CD133 reactivity of CD133^hi cells. Median and peak fluorescence channels were determined using the Stat program in CellQuest. FBS, fetal bovine serum; FACS, fluorescence-activated cell sorting.