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PCR amplicons resulting from kDNA PCR of 20 dogs (P01–P20). Positive samples of liver (P01L), spleen (P01S), and lymph nodes (P01N), of the index case (dog P01), and peripheral blood of the second diseased dog (P12) show the 145-bp-long fragments specific for L. infantum kDNA (indicated by the arrowhead; N, negative probe; P, positive probe).
