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Axon degeneration induces type-1 IFN genes in microglia. A, Real-time PCR shows induction of ISGs and IRFs in rat microglia cocultured with degenerating axons. All values are normalized to β-actin. Statistics: Student's t test, degenerating versus control at each time point. *p < 0.05; **p < 0.01; ***p < 0.001. Error bars indicate SEM. B, Immunostaining with antibody to IRF7 at 24 h.
