Figure 5.

Axonal debris clearance is attenuated in microglia derived from TRIF-knock-out mice. A, Microglia derived from wt and TRIF KO mice were cocultured with healthy or degenerating axons for 16 h. An identical experiment setup as in Figure 3 was used to quantify the extent of axon debris clearance by microglia. The dotted green traces outline axon bundle areas at time 0, whereas the dotted yellow traces outline the same region after 16 h. B, Quantification of axon debris clearance revealed a reduced clearance capacity for TRIF KO microglia compared with wt counterparts. One-way Tukey's ANOVA analysis, comparisons with WT degenerating condition; F value, 59.02. *p < 0.05; ***p < 0.001. Error bars indicate SEM.