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Summary of thermodynamic parameters estimated by non-linear least squares fit to Equation 1 describing a two-state equilibrium from the data in Figure 3, panels B, D and F.
| Method | Wavelength (nm) | Tm (°C) | ΔH°vH (kcal/mol) | A × 106 |
|---|---|---|---|---|
| Absorbance | 295 | 56.7 ± 0.5 | 18.7 ± 0.7 | 10.0 ± 0.3 |
| 260 | 51.0 ± 0.4 | 37.4 ± 1.7 | -17.6 ± 0.7 | |
| CD | 295 | 41.4 ± 0.4 | 32.0 ± 1.3 | -110 ± 4 |
| 260 | 33.1 ± 1.8 | 18.4 ± 2.2 | 800 ± 8 | |
| FRET | 570 | 32.1 ± 0.4 | 46.0 ± 0.4 | -50.0 ± 3.1 |
| 520 | 38.8 ± 0.4 | 21.0 ± 0.6 | 210 ± 5 |
