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Summary of thermodynamic parameters for melting of 143D in 50 mm NaCl assessed by different techniques derived from analysis of the data in Figure3, Panels A, C and E by GlobalWorks.
| Method | Std Dev of Fit | Tm1 (°C) | ΔH1 (kcal/mol) | Tm2 (°C) | ΔH2 (kcal/mol) | Tm3 (°C) | ΔH3 (kcal/mol) |
|---|---|---|---|---|---|---|---|
| Absorbance | 0.0006 | 24.5 ± 1.6 | 33.4 ± 0.2 | 51.8 ± 0.6 | 35.4 ± 1.3 | 70.1 ± 1.0 | 16.5 ± 0.4 |
| CD | 0.566 | 23.0 ± 0.7 | 21.7 ± 0.7 | 49.5 ± 0.5 | 34.5 ± 0.6 | 71.6 ± 1.6 | 17 ± 3 |
| FRET | 0.639 | 38.0 ± 0.4 | 24.6 ± 1.9 | 58.7 ± 0.2 | 14.4 ± 0.6 |
