Fig. 6.

Stage-specific reverse-transcription PCR of the HvMS1 anther-related gene. (A) PCR using primers based on the rice MS1 gene sequence (PTC1; Li et al., 2011) and RNA extracted from different spike sizes from barley variety Golden Promise. (B) Alpha tubulin PCR normalization of samples. Lane 1, 2–3 cm; lane 2, 3–4 cm; lane 3, 4–5 cm; lane 4, 5–6 cm; lane 5, >6 cm. Expression of the putative HvMS1 gene is clear in lanes 1 and 2; however, this expression is reduced or completely absent in lanes 3–5.