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. 2012 Mar 26;63(11):4151–4164. doi: 10.1093/jxb/ers098

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© 2012 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 2. — Functional analyses of plant RTHs in Arabidopsis. The seedling triple-response phenotype of ethylene-grown wild type (Col-0) and 35S:OsRTH1 transformation lines (A), (ETR1)4LOF and a mutant expressing 35S:OsRTH1 (B), etr1-7 and a mutant expressing 35S:OsRTH1 (C), and etr1-2 rte1-2 and a mutant expressing 35S:OsRTH1 (D). (E) The seedling triple-response phenotype of the wild type (Col-0) expressing 35S:OsRTH2 and 35S:OsRTH3. (F) Seedling triple-response phenotype of etr1-2 rte1-2 expressing 35S:OsRTH2 and OsRTH3. Leaf senescence phenotype of the wild type (Col-0) (G) and ethylene-insensitive etr1-2 (H); phenotype of wild type (Col-0), (ETR1)4LOF (J), etr1-2 rte1-2 (K), and etr1-7 (L) expressing 35S:OsRTH1. Air and ethylene indicate the phenotype of the same plants before and after the treatment, respectively. Chlorophyll a measurement (M) and SAG12 expression (N) of the wild type (Col-0), etr1-2, and 35S:OsRTH1 transformants in the corresponding mutation background as indicated. Error bars indicate the standard error (SE) for the means of five measurements. RT-PCR, analysis of the mRNA level of corresponding transgenes at the translational level. a (air) and b (ethylene) indicate a statistically significant difference (α=0.01) between the wild type and mutant or transformation lines.