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. 2012 Apr 17;63(11):4191–4212. doi: 10.1093/jxb/ers103

Fig. 4.

Fig. 4.

Relative expression of the CcUNK10 gene standardized with different reference genes. Transcript abundances were analysed by qPCR in leaves of drought-tolerant (14 and 120) and drought-susceptible (22) clones of C. canephora grown with (I) or without (NI) irrigation and strandardized independently with the CcUBQ10 (ubiquitin), CcADP1 (ADP ribosylation factor), CcACT1 (actin), CcCYN1 (cyclophilin), CcCTS1 (cathepsin), and CcGAPDH (glyceraldehyde-3-phosphate dehydrogenase) genes as references. Values are the mean of at least three technical repetitions ±SD. The significance of expression level differences between the treatments was evaluated using the pairwise Wilcoxon rank test (non-parametric test). Treatments sharing the same letter are not significantly different.