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. 2012 Apr 17;63(11):4191–4212. doi: 10.1093/jxb/ers103

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© 2012 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 5. — Expression profiles of genes down-regulated during drought acclimation. Gene expression was analysed in leaves of clones 14, 22, and 120 of C. canephora subjected (NI) or not (I) to drought. (A) Abundances of specific transcripts were analysed by northern blot and qPCR for CcCAB1, CcRBCS1, and CcACBP1. Transcript abundances of CcELIP1 and CcMET1 genes were monitored only by northern blot. For this analysis, total RNA stained with ethidium bromide was used to monitor the equal loading of the samples. Constitutive expression of the CcUBQ10 gene is also shown. (B) For other genes, transcript abundances were analysed by qPCR. The expression of the CcUBQ10 gene was used as a reference to measure the relative quantification that corresponds to the mean of three technical repetitions ±SD. The gene names are indicated in the histograms. Results are expressed using 14I as an internal calibrator. In each case, values of relative quantification correspond to the mean of at least three technical repetitions ±SD. Significance of expression level differences between the treatments was evaluated using the pairwise Wilcoxon rank test (non-parametric test). Treatments sharing the same letter are not significantly different.

Fig. 5. — Expression profiles of genes down-regulated during drought acclimation. Gene expression was analysed in leaves of clones 14, 22, and 120 of C. canephora subjected (NI) or not (I) to drought. (A) Abundances of specific transcripts were analysed by northern blot and qPCR for CcCAB1, CcRBCS1, and CcACBP1. Transcript abundances of CcELIP1 and CcMET1 genes were monitored only by northern blot. For this analysis, total RNA stained with ethidium bromide was used to monitor the equal loading of the samples. Constitutive expression of the CcUBQ10 gene is also shown. (B) For other genes, transcript abundances were analysed by qPCR. The expression of the CcUBQ10 gene was used as a reference to measure the relative quantification that corresponds to the mean of three technical repetitions ±SD. The gene names are indicated in the histograms. Results are expressed using 14I as an internal calibrator. In each case, values of relative quantification correspond to the mean of at least three technical repetitions ±SD. Significance of expression level differences between the treatments was evaluated using the pairwise Wilcoxon rank test (non-parametric test). Treatments sharing the same letter are not significantly different.