Table 2.

Identification of leaf coffee proteins presenting differential accumulation during drought acclimation

Protein names and corresponding peptide sequences obtained by MALDI-TOF-MS/MS are indicated and validated statistically by Scaffold Score expressed as a percentage (%). The observed isoelectric point (pI) and molecular weight (MW) were calculated from the 2-DE gels by ImageMaster Platinum 6.0 Software. Homology searches were done with the TBLASTN program (Altschul et al., 1990) using an E-value cut-off of 1e⁻¹⁰. GenBank (GB) and SGN (http://solgenomics.net, Mueller et al., 2005) accession numbers of ESTs and contigs containing the sequenced peptides are indicated.

Protein name	Gene name	Sequences	Scaffold Score (%)	Accession nos	pI/MW
Carbonic anhydrase	CcCA1	EKYETNPALFGQLAK	91	GT008701	6.85/23
		YMIVACADSR	85
		VNPSLILSLQPGDAFIVR	80
		SVANLVPPYDQLK	90
		HSEFGAAIEFAVLHLK	70
		VENIVVIGHSACGGIK	60
		GLMSIPEDGTTSTDFIEDWVK	90
		VKTEHGSKPLPEQIVLAE	95
Type-2C protein phosphatase (PP2C)	CcPP2C	KTSTSLMALSSPQLR	90	GW435032	5.26/22
		EEMEDDVIIVR	95	SGN-U631279
		ALEEAFESADMK	85
		EAGGWISNGR	85
Oxygen-evolving complex PSII (psbO)	CcPSBO	KDGIDYAAVTVQLPGGERV	90	GT647517	5.46/33
		RLTYDEIQSK	74	SGN-U629535
Oxygen-evolving complex PSII (psbP)	CcPSBP	WNPSKEVEFPGQVLR	90	GW488995	5.76/29
		YEDNFDSNSSVSVIIIPCDKK	50
		SITDYGPPEEFLSK	95
		VDYLLGK	52
		TEAEGGFEPNTVATANILEQETPIV	55
		TADGDEGGKHQLIR	90
Oxygen-evolving complex PSII (psbQ)	CcPSBQ	RFFIQALPPAGAAARA	90	GT645658	10.01/15
		DLDLPLKDR	74	SGN-U637442
		LKAELLR	62
		STPEAEKYYAATVSTLNDVLSK	95
		YDLNTVISAKPK	90
Small heat shock protein (sHSP)	CcHSP1	SAPVAPIGVLDRFPTAR	95	GW447897	5.87/22
		TVQQMMETMER	91
		NEDGEEEEKNEWSAK	90
		LMEDPFAYSGGWPSPLAPDTGGYSR	60
		GRTPWEIK	60
		ALPENAQF	70