Fig. 10.

Interaction between p24 proteins. (A) Western blot analysis showing the levels of p24β2 in membranes from the wild type (Col-0) or the p24δ5 knock-out mutant (p24δ5-1), using antibodies against the Nt or the Ct of p24β2. A 25 μg aliquot of protein was loaded in each lane. Western blot with an antibody against the plasma membrane (PM) ATPase was used as a loading control. (B) Immunoprecipitation of p24δ5 and p24β2 using affinity-purified antibodies against the Ct of both proteins or control beads, followed by SDS–PAGE and western blot with antibodies against p24δ5 (Nt) or p24β2 (Ct). The extract lane contains 20 μg of the membrane proteins used as input for the immunoprecipitation.