Fig. 2.

Characterization of p24 antibodies. (A) Characterization of antibodies against Arabidopsis p24 proteins. Protein extracts were obtained from membranes of Arabidopsis cell suspension cultures (C) or Arabidopsis roots (R), as described in the Materials and methods, and analysed by SDS–PAGE and western blotting with affinity-purified antibodies against the p24δ5 N-terminus, p24δ5 C-terminus, p24β2 N-terminus, and p24β2 C-terminus, or with the corresponding pre-immune sera. (B) Western blot analysis of membranes from wild-type (Col-0) or p24 knock-out mutants (p24δ5-1, p24δ4-1, or p24δ4δ5) using antibodies against the Nt or the Ct of p24δ5. A 25 μg aliquot of protein was loaded in each lane. Western blot with an antibody against the plasma membrane (PM) ATPase was used as a loading control.