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. 2012 Jun 30;18:1773–1786.

Table 1. Oligonucleotide primers used in semi-quantitative RT–PCR.

Gene Forward primer Reverse primer NCBI# Cycles ng RNA Annealing temp
beclin 1 CGGGAAGTCGCTGAAGACAG CCATCCTGGCGAGGAGTTTC NM_003766.3 30 100 55
atg14 GAGCGGCGATTTCGTCTACT CTGAAGACACATCTGCGGGG NM_014924.4 35 50 55
mtor TTCTGGTGCGACACCGAATC CATCGGGTTGTAGGCCTGTG NM_004958.3 30 100 55
rb1cc1/fip200 GGAGCTTGTGCACCTGAACT GAAGCACCCTCACCTGGTTTG NM_014781.4 35 50 55
ralb GCTCGTCGTGGGAAACAAGT TGACAAAGCAGCCCTTCCAC NM_002881.2 35 50 55
atg4a GAGTAAGGGCACCTCTGCCTA GTTCATTCGCTGTGGGGACT NM_052936.3 35 50 55
atg12 GAGGTCTGTAGTCGCGGAGA TGGATGGTTCGTGTTCGCTC NM_004707.3 30 100 55
map1lc3b /atg8 AAGTGGCTATCGCCAGAGTCG CTGAGATTGGTGTGGAGACGC NM_022818.4 25 100 55
rab7 GACACAGCAGGACAGGAACG TTGTACAGCTCCACCTCCGT NM_004637.5 25 100 55
fyco1 GAAGCTGAAGGCCACCCAAG GGGCATCTGACTTCTGCCAG NM_024513.3 35 50 55
bnip3l/nix ACTCGGCTTGTTGTGTTGCT TCCCTGCTGGTGTGCATTTC NM_004331.2 25 100 58
pink1 TCTGCAGTCCTCTGCTCACA GCTCATCCGTCACTTTCGCT NM_032409.2 35 50 55
p62 CTCACCGTGAAGGCCTACCT TAGCGGGTTCCTACCACAGG NM_003900.4 35 50 55
GAPDH CCACCCATGGCAAATTCCATGGCA TCTAGACGGCAGGTCAGGTCCACC NM_003900.4 35 50 60