Table 1. Primers used for PCR.
| Exon | Forward (5′-3′) | Reverse (5′-3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|---|
| 4 |
GGACTTAGGGTTTGATGACAG |
CCAGAAAGACCAGAGGCAC |
684 |
50 |
| 5 |
GAGATTGGCACAGGTTGG |
CATAAGTAGCATCGTTTACAGT |
423 |
60 |
| 5 a,6 |
AACGCCACTTTAAGCAAGGT |
GGAGGGCAGATGTTCTCAA |
620 |
52 |
| 7 |
AATCCACCCACTGTCCCG |
CCAGCCACCTTCATACCG |
542 |
60 |
| 8 |
TCAGGTAACTAACATCGCA |
GTTGACTGTACTTGGAAGAA |
719 |
53 |
| 9,10,11 |
GAGGTGGGAACCAGTTTGATG |
CAAGCCAATCTCTGTAGTGCG |
890 |
52 |
| 12 |
GAGGCTTGATACATAGGC |
CCATAAGACCAGGAGATT |
452 |
58 |
| 13(1) |
GTTTCTGAGGGTGCTACT |
TTGAATGGCTAACTGGG |
1519 |
48 |
| 13(2) |
CAGTTTCTGAAGGTGCTA |
TCCATCCAGTCTACATTG |
578 |
48 |
| 9 | GTAGTTCTGGCACAATATGG | GAGGTGCTTGTACAGAGTAC | 83 | 55 |
Sequence of oligonucleotides and annealing temperatures used for exon-by-exon PAX6 amplification.