Table 2. Sequence of forward and reverse primers used for PCR amplification.
Gene | Forward Primer (5′-3′) | Reverse Primer (5′-3′) | Expected PCR Product Size (bp) |
---|---|---|---|
ACTB | TGTCCACCTTCCAGCAGATGT | CACTCCCAGGGAGACCAAAA | 609 |
ADAM10 | AGGGAACGAGCAAGGGAAGG | GCCACCACGAGTCTGGATGA | 660 |
CALU | TGATGCCTTCTTGGGTGCTG | CCTGGCTTCTGCCTCTGCAT | 667 |
CTNNB1 | TGGCCATGGAACCAGACAGA | GGTCCCAGCGGTACAACGAG | 617 |
DCT | TGGTGAGAAGCGCTACCCTCAT | AGCCGGCAAAGTTTCCTGTG | 601 |
EDNRB | CCAACATGTGGCCCAGCCTA | TGAGGTGGGGTTGGAGGAAA | 231 |
PGCP | TCCCTGTGCTCTGGGAAAGC | GGCACGCCATCCTGGTATTC | 635 |
RAB27A | AACAAGCGGTTCTCTACCCTGT | TCCACACACCGTTCCATTCG | 617 |
TRPM1 | CAGGGTGGCGGATATTCCAA | CGCTGCCATCACAAATCACC | 664 |
TYRP1 | ACCGCTGTGGCTCATCATCA | TCCCCGTTGCAAAATTCCAG | 603 |