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. Author manuscript; available in PMC: 2012 Jul 17.
Published in final edited form as: Biochem J. 2011 Feb 1;433(3):505–514. doi: 10.1042/BJ20100791

Figure 4. Reduced hepatic NAD+ concentration correlates with increasing weight gain in HFD-fed mice.

Figure 4

C57BL/6 SVJ mice were fed on either CH or an HFD (45 kcal % fat) for 16 weeks (n = 4/group) and starved overnight prior to killing. (A) NAD+ levels were quantified using a colorimetric assay according to the manufacturer’s protocol. Values are means ± S.E.M., n = 6/group; #P < 0.05. (B) Hepatic NAD+ concentrations were compared with the net increase in body weight in mice fed on the HFD. NAD+ levels negatively correlated with the body weight increase (R2 = 0.88).