Figure 1.

Inducible RNAi System in the Moss P. patens.

(A) Scheme of the inducible RNAi system. The RNAi elements in the inducible RNAi vector construct were integrated into the genome of a transgenic line coexpressing GFP-tubulin and histone-RFP. Transcription followed by formation of dsRNA targeting histone-RFP, and a target gene was induced upon addition of β-estradiol to the medium. Supplemental Figure 1A online shows a more detailed map of this construct.

(B) Selection of inducible profilin RNAi lines. After selection of seven stable transformants (RNAi candidate lines), cells were treated with β-estradiol for 5 d, followed by measurement of histone-RFP intensity and cell length (the second cell from the apical tip was selected for length measurement) (error bars indicate sd; n = 24 to 36).

(C) Appearance of shorter cells after inducible profilin RNAi (line #10). Enlarged images are displayed at the bottom. Bar = 100 µm.