Figure 2.

ann1 Root Hair Apical PM Retains the Constitutive HACC Conductance.

Representative currents recorded in the whole-cell patch clamp configuration from an ann1 root hair apical spheroplast PM showing the HACC conductance that is a characteristic of the root hair apex. No OH^• were used in this experiment, and Ba²⁺ replaced Ca²⁺ in the bathing medium. Current flowing below the time axis is cation entry into the protoplast. Bathing solution comprised 10 mM BaCl₂ and 2 mM MES-Tris, pH 6, adjusted to 275 mOsM with d-sorbitol. Pipette solution comprised 0.5 mM CaCl₂, 8.5 mM Ca(OH)₂, 2 mM MgATP, 0.5 mM Tris-ATP, 10 mM BAPTA (final free Ca²⁺, 1 μM), and 15 mM HEPES-Tris, pH 7.3, adjusted to 275 mOsM with d-sorbitol.