Figure 1.

Expression Pattern of NTRC in Arabidopsis.

(A) qPCR analysis of NTRC transcripts in different organs of Arabidopsis wild-type plants. The amount of transcripts in each organ was represented as arbitrary units relative to the level in leaves, which was set to 1.0. Analysis was performed three times on two independent biological samples, and the mean values ± se are indicated.

(B) The amount of NTRC protein was determined by immunoblot probed with an anti-NTRC polyclonal antibody. Samples (30 μg of protein) were subjected to SDS-PAGE, transferred onto nitrocellulose membranes, and probed with anti-NTRC or anti-NTRB antibodies to test even protein loading, as indicated. Arabidopsis wild-type and ntrc mutant plants were grown under long-day conditions for 48 d. RNA, for qPCR, and protein, for immunoblot analysis, were extracted from leaves (L), roots (R), stems (S), and flowers (F). WT, wild type.