Subcellular Localization of NTRC in Arabidopsis Plants.
(A) to (D) Confocal microscopy micrographs of mesophyll ([A] and [B]) and guard cells ([C] and [D]) of Arabidopsis plants transformed with the NTRC-GFP fusion protein expressed under the CaMV 35S promoter ([A] and [C]) or the NTRC gene promoter ([B] and [D]). Plants were grown for 18 d.
(E) to (J) Subcellular localization of NTRC in nonphotosynthetic tissues of Arabidopsis. Confocal microscopy micrographs showing plastid localization of NTRC in roots of 5-d-old seedlings with the NTRC-GFP fusion protein expressed under the CaMV 35S promoter (E) or the NTRC gene promoter (F) and a root of plants expressing the GFP protein not fused to NTRC
(G). Plastid localization of NTRC in petal (H) and anther (I) of 48-d-old plants and hypocotyl of 5-d-old Arabidopsis seedlings grown under darkness (J) and expressing the NTRC-GFP fusion protein under the 35S promoter.
Red, chlorophyll autofluorescence; green, GFP fluorescence. Arrow indicates a chloroplast stromule. Sizes of bars are as indicated.