Figure 4.

Unbalanced Redox Status of 2-Cys PRXs in Photosynthetic and Nonphotosynthetic Tissues of ntrc Mutant Plants.

Protein extracts from leaves (L), roots (R), stems (S), and flowers (F) of Arabidopsis wild-type (WT) and ntrc mutant plants were subjected to SDS-PAGE under reducing (7.5 μg of protein loaded) (A) or nonreducing conditions (15 μg of protein loaded) (B), as indicated, electrotransferred to nitrocellulose sheets, and probed with anti-2-Cys PRX antibodies. “mon” indicates the monomeric and “dim” the dimeric form of the enzyme. Molecular mass markers (kD) are indicated on the left.