Figure 5.

Effect of the Presence of NTRC in Leaves and Roots on the Redox Status of 2-Cys PRX.

(A) Immunoblot analysis of the content of NTRC in leaf (30 μg of protein) and root (50 μg of protein) extracts from the wild type (WT), ntrc mutant, ntrc mutant transformed with the empty vector (ntrc-ev), and transgenic lines expressing NTRC under the CaMV 35S, Rbcs, Pht1,3, and Pht1,2 promoters, as indicated, in the ntrc mutant background. Proteins were subjected to SDS-PAGE under reducing conditions, electrotransferred to nitrocellulose sheets, and probed with anti-NTRC antibodies.

(B) Aliquots (15 μg of protein) of the same protein samples indicated above were subjected to SDS-PAGE under nonreducing conditions, electrotransferred to nitrocellulose sheets, and probed with anti-2-Cys PRX antibody. “mon” indicates the monomeric and “dim” the dimeric form of the enzyme. Molecular mass markers (kD) are indicated on the left.