Figure 4.

Transient Expression of EGFP Fusion Proteins with Full-Length or Partial Sequences of Toc159.

Isolated Arabidopsis protoplasts were transfected with the EGFP fusion constructs for transient protein expression driven by the constitutive 35S promoter (left panels). For each construct, representative images of EGFP and chlorophyll fluorescence and a merge of the two channels are shown in the middle panel. The subcellular localization was confirmed by immunoblot analysis with an anti-EGFP antibody after subfractionation of the total protoplasts or purified chloroplasts into pellet (P) and soluble (S) fractions and analyzed by densitometric quantification (right panels). Detection with an antibody against Rubisco large subunit (RbcL) served as loading controls for the soluble fractions. Bar = 10 μm.

(A) Full-length Bs Toc159 fused to the C terminus of EGFP.

(B) Full-length Bs Toc132 fused to the C terminus of EGFP.

(C) C-terminally truncated Bs Toc159 fused to the C terminus of EGFP

(D) C-terminally truncated Bs Toc132 fused to the C terminus of EGFP.

(E) EGFP control in null vector.

(F) The CT of Bs Toc159 fused to the C terminus of EGFP.

(G) The CT of Bs Toc132 fused to the C terminus of EGFP.

[See online article for color version of this figure.]